Gidan wutar lantarki a kasan duniyar anti-tsufa ——NMNH

01.NMNHyana haɓaka matakan NAD + da NADH ta salula. Masu bincike sun kwatanta tasirin NMN da NMNH akan haɓaka matakan NAD + na salula. Jiyya tare da NMNH (100 μM) ya karu matakan NAD + ta salula ta hanyar 5-ninka a cikin kwayoyin HepG2, yayin da 100 μM NMN kawai ya karu matakan NAD + kadan. Baya ga sel HepG2, masu binciken sun kuma tabbatar da cewa NMNH sun haɓaka NAD + a cikin ƙwayoyin ES-2 da sel na 3T3-L1, waɗanda su ne ƙwayoyin ovary da aka samu na ɗan adam da ƙwayoyin fibroblast-embryoblast na linzamin kwamfuta, bi da bi. Kuma masu binciken sun kuma gano cewa NMNH ya karu da matakan NAD + na salula a cikin kwayoyin 786-O a cikin lokaci-da kuma dogara da hankali. Masu binciken sun auna NADH ta hanyar amfani da nau'in spectrometry kuma sun gano cewa matakan NADH a cikin kwayoyin HepG2 da aka yi wa NMNH sun fi sau 2.5 fiye da kwayoyin da ba a kula da su ba da kuma NMN, kuma an gano matakan NADH masu girma a cikin kwayoyin 786-O na NMNH. Mun kuma gano cewa 340 mg / kg NMNH magani ya karu da matakan NADH a cikin hanta linzamin kwamfuta ta hanyar 3-ninka, yayin da 340 mg / kg NMN magani ya karu matakan NADH da 1.7-ninka.

Hoto 3.4. NMNH yana ƙara matakan NMNH

02NMNH yana hana glycolysis da sake zagayowar TCA. Tarin NADH yana rage jinkirin glycolysis da sake zagayowar TCA. Don bincika amsawar rayuwa na sel zuwa jiyya na NMNH, masu binciken sunyi nazarin metabolomic naNMNH-da Kwayoyin da aka yi wa NMN magani. An gano cewa jiyya na NMNH ba kawai ya rage matakan glycolysis masu tsaka-tsaki ba, amma kuma ya rage matakan tsaka-tsakin zagaye na TCA. Waɗannan sakamakon suna nuna cewa tarin NADH da NMNH ke samarwa yana hana glycolysis na salula da zagayowar TCA.

Hoto 6. Binciken Metabolomics

03.NMNHyana hana haɓakar ƙwayoyin cuta Masu bincike sun bincika tasirin NMNH akan haɓakar tantanin halitta kuma sun gano cewa jiyya na NMNH ya hana haɓakar kwayar cutar HepG2 a mafi girma fiye da 250 μM. Masu binciken sunyi nazarin tsarin kwayar halitta a cikin kwayoyin HepG2 da aka bi da su tare da 1 mM NMNH ko NMN na tsawon sa'o'i 12 kuma sun gano cewa NMNH ya haifar da kama hawan tantanin halitta, yayin da NMN ba shi da wani tasiri a kan tsarin tantanin halitta. Bugu da kari, masu binciken sun kuma gano cewa 500 μM NMNH gaba daya sun toshe ci gaban kwayoyin 786-O; 250 μM NMNH zai iya hana haɓakar ƙwayar sel yadda ya kamata a cikin sel HK-2.

Hoto 7.8.NMNH yana hana ci gaban kwayar halitta a cikin vitro

04.NMNHMasu bincike na aminci sun gudanar da gwaje-gwaje akan ko yawan allurai na NMNH yana haifar da ciwon hanta. Jiyya na C57BL / 6J mice maza tare da 50, 100, 500, ko 1000 mg/kg NMNH ta hanyar allurar intraperitoneal kowace rana har tsawon mako guda ya nuna babu raguwa a cikin kwayoyin alanine aminotransferase (ALT) da matakan aspartate aminotransferase (AST). Girma, yana nuna cewa NMNH ba hepatotoxic ba ne a babban allurai. Bugu da ƙari, masu binciken sun gano cewa maganin NMNH ya karu da abun ciki na NAD + a cikin hanta na linzamin kwamfuta a cikin hanyar da ta dace. Jiyya tare da 1000 mg/kg NMNH ya haɓaka matakan NAD + a cikin hantar linzamin kwamfuta fiye da sau 10.

Hoto 10.NMNHyana ƙara matakin NAD+ a cikin hantar mice

Nazarin ya nuna cewa NMNH shine mafi kyawun NAD + - haɓaka kari fiye da NMN duka a cikin vitro da in vivo; Bugu da ƙari, NMNH yana ƙara rage matakan NAD (NADH) a cikin sel da hanta linzamin kwamfuta. Binciken metabolomic ya nuna cewa NMNH yana hana glycolysis da sake zagayowar TCA. Gwaje-gwajen in vitro sun nuna cewa NMNH yana haifar da kama hawan kwayar halitta kuma yana hana ci gaban tantanin halitta. Haɗe tare, wannan binciken ya nuna cewa NMNH shine mai haɓaka NAD + mai ƙarfi kuma yana hana glycolysis, sake zagayowar TCA, da haɓakar tantanin halitta.

Sunan Sinanci na NMNH shine "rage β-nicotinamide mononucleotide", wanda shine raguwar nau'in NMN kuma foda ne mai launin rawaya. Tsarin kwayoyin halitta: C11H17N2O8P Nauyin kwayoyin halitta: 336.23g / molCAS A'a.: 108347-85-9 Sabbin wallafe-wallafen bincike sun nuna cewa NMNH yana ƙara matakan NAD + da sauri kuma a mafi girma maida hankali, a matsakaita 5 sau na NMN.