In order to compare the effects of NMNH and NMN, the researchers injected PBS and 250 mg/kg of NMN and NMNH into C57BL/6N mice, respectively, and then conducted sequential blood sampling. The results showed that NMNH significantly increased NAD levels in the blood, to a much greater extent than NMN. NAD was measured on the collected tissues through the enzyme cycle method. The results showed that NMNH increased the NAD levels in various tissues, and the effect was higher than that of NMN. It had the most obvious effect on the liver and kidneys, with the liver increasing by more than 5 times and the kidneys increasing by more than 5 times. 2 times.
NMNH was supplemented during the reoxygenation phase, and the expression of renal tubular injury marker KIM-1 was detected. Reoxygenation in TEC induced a sharp increase in KIM-1 expression, which decreased sharply after NMNH treatment, indicating that administration of NMNH can effectively and significantly reduce the damage to renal tubular epithelial cells during hypoxia and reoxygenation.
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